IL-33/ST2 Activation Is involved in Ro60-Regulated Photosensitivity in Cutaneous Lupus Erythematosus

Figure 2

IL-33 promotes proinflammatory cytokine secretion and Ro60 expression in murine KCs upon UVB irradiation. In vitro, the PAM212 KCs were subjected to UVB irradiation, either alone or combined with IL-33 and bovine serum albumin (BSA) stimulation. (a) qRT-PCR determination of ST2, SOX17, CCL2, RANTES, ICAM-1, MCP-1, and IP-10 mRNA expression in KCs. (b) qRT-PCR detection of Ro60 mRNA in KCs. (c) Western blotting detection of Ro60 protein in cell lysates and supernatants of KCs. (d) Densitometry analysis of Western blotting bands of supernatant Ro60. (e) Densitometry analysis of Western blotting bands of cell lysate Ro60. (f) Western blotting analysis of Ro60 in cells transfected with ST2 siRNA or control siRNA and subjected to UVB and IL-33 treatments. (g) Densitometry analysis of Western blotting bands of Ro60 in ST2 siRNA- or control siRNA-transfected cells with UVB and IL-33 treatments. (h, i) Immunofluorescence analysis of ST2 and Ro60 in KCs subjected to different treatments. Data were obtained from three experiments conducted independently. Data points and error bars denote . . , , and . KC: keratinocyte.

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