IL-33/ST2 Activation Is involved in Ro60-Regulated Photosensitivity in Cutaneous Lupus Erythematosus

Figure 6

SOX17 is pivotal in IL-33’s effect on KCs. (a) Detection of SOX17, NF-κB, PI3K, AKT, and Ro60 mRNA expression in KCs following IL-33 treatment by qRT-PCR. (b) Western blot determination of SOX17, NF-κB, NF-KBP65, PI3K, p-PI3K AKT, p-AKT, and Ro60 expression in KCs following IL-33 irradiation. (c) qRT-PCR determination of SOX17 expression in KCs after SOx17 siRNA transfection. (d) Western blot determination of SOX17 expression in KCs after SOX17 siRNA transfection. (e) qRT-PCR determination of SOX17, CCL2, and ICAM-1 mRNA expression in KCs following IL-33 stimulation. (f) Western blot determination of SOX17, NF-κB, NF-KBP65, PI3K, p-PI3K AKT, p-AKT, and Ro60 expression in KCs following SOX17 siRNA transfection and IL-33 treatment. Data were obtained from three experiments conducted independently. Data points and error bars denote . , . KC: keratinocyte.

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