Anti-Inflammatory Activity of Ferula assafoetida Oleo-Gum-Resin (Asafoetida) against TNF-α-Stimulated Human Umbilical Vein Endothelial Cells (HUVECs)

Figure 1

Cell viability, cell adhesion, and intracellular ROS production assay. (a) Effect of EEA on the viability of HUVECs that were treated at various EEA concentrations (0, 31, 62, 125, 250, and 500 μg/ml) for 1 day. MTT test determined HUVECs’ viability. The cell viability did not show significant differences up to 500 μg/ml. (b) HUVECs were treated at various TNF-α amounts (0, 0.1, 1, 10, and 100 ng/ml) for 24 h followed by an MTT reagent to evaluate cell viability. The difference was not statistically significant in cell viability at different concentrations. (c) HUVECs were treated under different TNF-α levels (10, 20, and 40 ng/ml) for 6, 12, and 18 h, and a PBMC adhesion assay was carried out. (d) Cells were treated with distinct concentrations of TNF-α (10, 20, and 40 ng/ml) for half a day, and intracellular ROS production was assessed. Stimulation with TNF-α at 20 ng/ml significantly increased the PBMC adhesion and ROS production after 12 h. Every value is entered as the from at least separate triple tests. and versus control. N.S.: not significant.

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