Research Article

Anti-Inflammatory Activity of Ferula assafoetida Oleo-Gum-Resin (Asafoetida) against TNF-α-Stimulated Human Umbilical Vein Endothelial Cells (HUVECs)

Figure 2

EEA reduces TNF-α-stimulated ROS production and PBMC adhesion to HUVECs. Target cells were incubated with two concentrations of EEA (125 and 250 μg/ml) for 6 h, followed by treatment along with TNF-α (20 ng/ml) for 12 h. (a) DCFH-DA probes assessed ROS production. Fluorescent microscopy showed decreased ROS production in Assafoetida pretreatment. (b) The adhesive function of PBMCs for HUVECs was assessed. Fluorescent microscopy showed decreased adhesion in EEA pretreatment. The data reflects the (). and opposed to control and TNF-α-treated groups. N. S: not significant.
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