Research Article
CpG Oligodeoxynucleotides Inhibit RANKL-Induced Osteoclast Formation by Upregulating A20 Deubiquitinase in RAW 264.7 Cells
Figure 1
The inhibitory effect of CpG-ODN on osteoclastogenic molecules. (a) c-Fos mRNA expression in RAW 264.7 cells stimulated with either RANKL (100 ng/ml) for 6 days or CpG-ODN (1 μM) for 4 days and both. (b) c-Fos, p-IκBα, and p-NF-κB protein expressions in RAW 264.7 cells stimulated with either RANKL or CpG-ODN and both. (c, d) mRNA and protein expression of osteoclast-specific molecules such as NFATc1, TRAP, cathepsin K, and CA-II in RAW 264.7 cells incubated with either RANKL or CpG-ODN and both. Abbreviation: NFATc1: nuclear factor of activated T cells c1; TRAP: tartrate-resistant acid phosphatase; CA-II: carbonic anhydrase-II. Error bars represent standard deviation of the measurement of three separate samples ( and ).
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