MFG-E8 Knockout Aggravated Nonalcoholic Steatohepatitis by Promoting the Activation of TLR4/NF-κB Signaling in Mice

Figure 7

Effect of MFG-E8 knockout on TLR4/NF-κB signaling pathway in the liver of NASH mice. The indicated proteins in whole cell lysates, cytoplasmic extraction, and nuclear extraction from the liver tissues were detected by Western blotting, respectively. Representative Western blotting (a) and quantification (b) of TLR4, p-IRAK1, p-p38, p38, p-p65, p65, and GAPDH protein levels in the liver. Representative Western blotting (c) and quantification (d) of p-IKKβ, IKKβ, p-IκBα, IκBα, and GAPDH in the whole cell lysates, p65, and β-actin in the cytoplasmic extraction, as well as p65 and Lamin B1 in the nuclear extraction from liver tissues. Data were expressed as , , ^#, ^## compared with CD-WT group, , , compared with MCD-WT group.

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