MFG-E8 Knockout Aggravated Nonalcoholic Steatohepatitis by Promoting the Activation of TLR4/NF-κB Signaling in Mice

Figure 8

TLR4 antagonist rescued MFG-E8 knockout-enhanced TG synthesis and proinflammatory cytokine production in primary hepatocytes stimulated by palmitic acid (PA). The primary hepatocytes separated from WT or MFG-E8 knockout mice were stimulated by PA (0.5 mM) with or without TLR4 specific antagonist TAK-242 (10 nM) for 24 h, the lipid droplet was evaluated by Oil Red O staining (a), TG content was assayed by a commercial kit (b), and TNF-α (c) and IL-1β (d) protein levels in the supernatants were measured by ELISA. Data were expressed as , , ^#, ^## compared with WT + TAK-242 group, , compared with KO + PBS group.

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