Anti-Inflammatory Activity of Glabralactone, a Coumarin Compound from Angelica sinensis, via Suppression of TRIF-Dependent IRF-3 Signaling and NF-κB Pathways

Figure 2

Inhibitory effects of glabralactone on NO production and iNOS expression in LPS-stimulated RAW 264.7 cells. (a) RAW 264.7 cells were stimulated with LPS (1 μg/mL) in the presence or absence of glabralactone. After 20 h, the nitrite concentrations were analyzed in the cultured media. The values are expressed as the . was considered statistically significant. (b) Cell viability was determined using MTT assay. All experiments were performed in triplicate. The data are expressed as . (c) The regulation of iNOS protein expression by glabralactone was determined. Cells were treated with LPS (1 μg/mL) and glabralactone (0–20 μM) for 16 h. After incubation, Western blotting was performed as described in Materials and Methods. Data are representative of three separate experiments. β-Actin was used as an internal standard. Compounds were dissolved in 100% DMSO, and the final concentration of DMSO was adjusted to 0.2% (a), 0.5% (b), and 0.1% (c).

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