Endoplasmic Reticulum Stress in Colonic Mucosa of Ulcerative Colitis Patients Is Mediated by PERK and IRE1 Pathway Activation

Figure 3

Activation of UPR-responsive genes and chaperone modulation. (a) Transcriptional levels of STC2, ATF3, and DDIT3 are shown as a fold change; , . (b) Quantitative analysis of immunohistochemistry staining for DDIT3 of both groups. For UC, ; for CTR, ; . (c) Immunohistochemical analysis of DDIT3 was performed on paraffin-embedded slides from intestinal mucosa of both UC and CTR groups. The arrows indicate DDIT3-positive cells, which are shown in brown. The white arrows indicate positive epithelial cells, and the black arrows signalize positive cells from the lamina propria. Scale bars: 50 μm. (d) Transcriptional levels of the chaperones DNAJC3, CALR, HSPA5, and HSP90B1 are shown as fold change, . (e, f) Immunohistochemical analysis of GRP78 and GRP94 was performed on paraffin-embedded slides from intestinal mucosa of both groups. The arrows indicate GRP78- and GRP94-positive cells, which are shown in brown. The white arrows indicate positive epithelial cells, and the black arrows signalize positive cells from the lamina propria. Scale bars: 50 μm. (g, h) Quantitative analysis of immunohistochemistry staining for GRP78 and GRP94 of both groups. (g) For UC, ; for CTR, ; . (h) For UC, ; for CTR, ; . CTR = control; UC = ulcerative colitis.

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