mTOR Modulates the Endoplasmic Reticulum Stress-Induced CD4⁺ T Cell Apoptosis Mediated by ROS in Septic Immunosuppression

Figure 3

Expression levels of mTOR pathway proteins, ERS-associated proteins, and apoptosis-associated proteins in WT, CLP, and CLP+4-PBA mouse groups. After purifying the CD4⁺ T cells from mouse spleen lymphocytes, whole cell lysates were assessed for the protein expression of (a) patterns of mTOR pathway proteins, including mTOR, P-mTOR, downstream effectors p70s6k, p-p70s6k, 4EBP, and P-4EBP; (b) ERS-associated proteins, including GRP78 and CHOP; (c) apoptosis-associated proteins, including caspase-3, Bax, and Bcl-2. The protein expression was detected by immunoblotting. Data are . biologically independent experiments (one-way ANOVA Tukey’s post hoc test). , , and .

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