mTOR Modulates the Endoplasmic Reticulum Stress-Induced CD4⁺ T Cell Apoptosis Mediated by ROS in Septic Immunosuppression

Figure 5

Role played by mTOR in ERS and CD4⁺ T cell apoptosis. CD4⁺ T cell apoptosis percentage was assessed by flow cytometry (a). Left panel: gating strategy for apoptosis cells; right panel: percentage of apoptotic cells in the left panel. Graphs show , four mice per group. mRNA expression level of BIM in CD4⁺ T cells was analyzed by RT-PCR (b). Representative images of ER in CD4⁺ T cells from CLP, LCK-TSC1+CLP, and Lck-mTOR+CLP mice, as observed by electron microscopy (c). Data are , , analyzed using one-way ANOVA. Blue arrows represent the normal-sized ER. Red arrows represent dilation of the ER. .

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