mTOR Modulates the Endoplasmic Reticulum Stress-Induced CD4⁺ T Cell Apoptosis Mediated by ROS in Septic Immunosuppression

Figure 7

Role played by ROS in ERS and CD4⁺ T cell apoptosis. The apoptosis percentage of CD4⁺ T cell from WT, CLP, CLP+CIRP-Ab, and CLP+NAC mice was flow cytometrically determined (a). Left panel: gating strategy for apoptosis cells; right panel: percentage of apoptotic cells in the left panel. Data represent the . Graphs show , four mice per group. CD4⁺ T cells were treated with CM-H2DCFDA for 45 min prior to ROS analysis by flow cytometry (b). Representative images of ER in CD4⁺ T cells from CLP, CLP+CIRP-Ab, and CLP+NAC mice, as observed by electron microscopy (c). Data are of 4 independent experiments (one-way ANOVA Tukey’s post hoc test). Blue arrows indicate normal-sized ER. Red arrows indicate dilatation and vesiculation of the ER. Magnification, ×20,000; .

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