Interference of Interleukin-1β Mediated by Lentivirus Promotes Functional Recovery of Spinal Cord Contusion Injury in Rats via the PI3K/AKT1 Signaling Pathway

Figure 4

Preparation of IL-1β-RNAi-LV recombinants. (a) Schematic representation of IL-1β-RNAi-LV, in which, enhanced red fluorescent protein (mCherry FP) as the reporter gene was inserted into the plasmid. The framework also contains the antibiotic ampicillin and the plasma cloning vector pUC Ori, which were used as promoters for vector expression. (b) Screening of effective interference fragment for IL-1β inhibition showed that F3 exhibited the most effective interference of the three tested fragments. (c) Fluorescent image of IL-1β-RNAi-LV transfected into 293 T cells.

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