Interleukin-19 Aggravates Pulmonary Fibrosis via Activating Fibroblast through TGF-β/Smad Pathway

Figure 2

IL-19 promotes proliferation and invasion, while represses apoptosis of lung fibroblasts. (a) Lung fibroblasts appreciate rates were assessed by CCK-8 assay at 0 h, 24 h, 48 h, and 72 h with different IL-19 concentration of 0, 10, 100, and 200 ng/ml, and combination TGF-β1 (5 ng/ml) of different IL-19 concentration (0, 10, 100 and 200 ng/ml), and 72 h cell appreciate rates were analyzed (d and e). (b) Lung fibroblasts were, respectively, treated with TGF-β1 (5 ng/ml), IL-19 (100 ng/ml), IL-19 + TGF-β1 (), cell invasions were evaluated by transwell assay, and numbers of migrated cells were quantified (f). (c) The degrees of apoptosis were assessed by flow cytometry using propidium iodide and Annexin V staining, and apoptosis rates were calculated (g). , , , and .

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