Interleukin-19 Aggravates Pulmonary Fibrosis via Activating Fibroblast through TGF-β/Smad Pathway

Figure 5

IL-19 aggravated lung fibrosis in wild-type and murine bleomycin fibrosis models. Schematic showing the induction of pulmonary fibrosis of lung collagen visualized by histopathological analysis after IL-19 and BLM challenge. After treatment with a single dose of BLM (2.5 mg/kg) or IL-19 (200 ng/kg) and combination of IL-19 and BLM, C57BL/6 mouse lungs were isolated and subjected for histopathology. (a) Representative pictures (20×) of H&E-stained, Masson’s trichrome-stained sections and immunohistochemistry for α-SMA in lung sections. Effect of IL-19 on mice lung tissues were reflected by changes in the Ashcroft histology score (b) and lung hydroxyproline content (c). (d) Table represents the semiquantitative evaluation of protein expression (α-SMA) in specified treatment groups. In b–c, each symbol represents an individual mouse, a total of 5 to 7 mice per group. Scale bars, 200 μm. , , , and .

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