Chlorpromazine and Promethazine (C+P) Reduce Brain Injury after Ischemic Stroke through the PKC-δ/NOX/MnSOD Pathway

Figure 2

C+P attenuated NOX activity and ROS level postreperfusion. Quantitative analysis of (a) NOX activity and (b) ROS level as determined in ischemic brain tissue. NOX activity was higher at 24 hours of reperfusion, and ROS levels were also higher at 6 and 24 hours of reperfusion. Decreased NOX activity levels at 24 hours of reperfusion and ROS at 6 and 24 hours of reperfusion were observed in C+P groups regardless of temperature control as well as in PKC-δ or NOX inhibitor groups (). versus the sham group; ^#, ^### versus the I/R group. Data is shown as the . Quantitative analysis of (c) ROS level in SHSY5Y cell was measured. C+P administration lowered the OGD-induced elevation of ROS at 6 and 24 hours of reoxygenation. , versus the OGD group; ^# versus the OGD/C+P group. Results are shown as of three independent experiments.

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