Chlorpromazine and Promethazine (C+P) Reduce Brain Injury after Ischemic Stroke through the PKC-δ/NOX/MnSOD Pathway

Figure 3

C+P decreased NOX subunit protein levels after stroke in ischemic brain tissue. (a) gp91^phox, (b) p67^phox, (c) p47^phox, and (d) p22^phox assessed via the Western blot technique at 6 and 24 hours of reperfusion. Levels of gp91^phox, p67^phox, p47^phox, and p22^phox rose after I/R but decreased after C+P treatment and administration of the PKC-δ or NOX inhibitor at 24 hours of reperfusion, with the exception of p22^phox in the C+P at the 37°C group at 24 hours of reperfusion. There was a larger decrease in p22^phox expression in the non-temperature-controlled C+P versus the temperature-controlled C+P group at 24 hours of reperfusion (). and versus the sham group; ^#, ^##, and ^### versus the I/R group; ^&&& versus the C+P group. Results are shown as . The representative immunoblots are visualized.