Chlorpromazine and Promethazine (C+P) Reduce Brain Injury after Ischemic Stroke through the PKC-δ/NOX/MnSOD Pathway

Figure 7

C+P facilitated the expression of MnSOD after cerebral ischemia or OGD/R. (a) Representative bands of MnSOD in brain tissue as identified using Western blot at 6 and 24 hours of reperfusion. β-Actin served as an internal control. Semiquantitative levels of MnSOD are displayed in bar graphs, identified using band density analysis. MnSOD protein levels decreased after I/R. C+P administration significantly accelerated MnSOD expression at 6 hours of reperfusion (). versus the sham group; ^### versus the I/R group. The data is presented as . (b) Representative bands of MnSOD expression in SHSY5Y cells as identified using Western blot at 6 and 24 hours of reoxygenation. β-Actin protein served as an internal control. Bar graphs show semiquantitative levels of MnSOD as identified using band density analysis. MnSOD protein levels decreased at 24 hours of reoxygenation. However, C+P therapy elevated MnSOD expression at 6 and 24 hours of reoxygenation. versus the OGD group; ^# versus the OGD/C+P group. Results are shown as of three independent experiments.

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