Human Neutrophil Defensins Disrupt Liver Interendothelial Junctions and Aggravate Sepsis

Figure 1

Administration of a high dose of HNP-1 aggravates sepsis outcome. Wild-type mice were intraperitoneally administered a high dose of HNP-1 (10 mg/kg body weight), a low dose of HNP-1 (0.5 mg/kg body weight), or PBS 6 hours after CLP or sham operation. (a) Survival over time. mice in each CLP group, and mice in each sham-operated group. (b) Clinical signs at 24 hours and 48 hours after CLP. The data shown are the of 6-7 mice in each CLP group and 4 mice in each sham-operated group. (c) Representative images of hematoxylin and eosin-stained liver sections from mice 48 hours after CLP or sham operation. Scale bars, 50 μm. (d) Quantification of the liver injury score based on the hematoxylin and eosin-stained liver sections in (c). The results shown are the of 5 mice in each CLP group and 4 mice in each sham-operated group. (e) Representative images of hematoxylin and eosin-stained lung sections from mice at 48 hours after CLP or sham operation. Scale bars, 50 μm. (f) Quantification of the lung injury score based on the hematoxylin and eosin-stained lung sections in (e). The results shown are the of 5 mice in each CLP group and 4 mice in each sham-operated group. , , and .

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