Gasdermin D Deficiency Does Not Protect Mice from High-Fat Diet-Induced Glucose Intolerance and Adipose Tissue Inflammation

Figure 3

Liver inflammation and metabolism in wild-type (WT) and gasdermin D knockout (GSDMD KO) mice fed a high-fat diet (HFD). (a) Representative western blots and quantitative analysis of GSDMD in liver. (b) IL-1β protein expression in liver was measured by ELISA assay. (c) Representative western blots and quantitative analysis of pro-caspase1 and caspase1 in liver. The expression level of protein was normalized using β-tubulin. (d–h) Real-time PCR analysis of genes in the liver. The expression level of each target was normalized using 18S rRNA. (i) GSH concentration in liver was evaluated by GSH Detection Assay kit. vs. same genotype mice fed a NCD, ^† vs. WT NCD group, and ^# vs. WT HFD group.

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