Gasdermin D Deficiency Does Not Protect Mice from High-Fat Diet-Induced Glucose Intolerance and Adipose Tissue Inflammation

Figure 4

Adipose tissue inflammation and metabolism in wild-type (WT) and gasdermin D knockout (GSDMD KO) mice fed a high-fat diet (HFD). (a, c, d, g, h, and i) Real-time PCR analysis of genes in visceral adipose tissue (VAT). The expression level of each target was normalized using 18S rRNA. (b) IL-1β protein expression in VAT was measured by ELISA assay. (e, f, and j) Representative western blots and quantitative analysis of protein expression in VAT. The protein expression level was normalized using β-actin. (k) GSH concentration in VAT was evaluated by GSH Detection Assay kit. vs. same genotype mice fed a NCD, ^† vs. WT NCD group, and ^# vs. WT HFD group.

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