Gasdermin D Deficiency Does Not Protect Mice from High-Fat Diet-Induced Glucose Intolerance and Adipose Tissue Inflammation

Figure 6

Macrophage activation in wild-type (WT) and gasdermin D knockout (GSDMD KO) mice fed a high-fat diet (HFD). (a and c) Bone marrow-derived macrophages were isolated from WT and GSDMD KO mice fed a normal chow diet (NCD) or HFD. Bone marrow-derived macrophages (BMDMs) were treated with LPS, and gene expression of GSDMD and IL-1β was measured by real-time PCR. Gene expression levels were normalized to 18S rRNA. (b) Representative western blot image and quantification of NLRP3 after LPS and ATP treatment. Protein expression levels were normalized to β-tubulin. (d) Secretion of IL-1β protein from BMDMs was measured by ELISA after treatment with LPS and ATP. vs. control, ^† vs. LPS, ^‡ vs. NCD LPS+ATP, and ^ɤ vs. HFD LPS+ATP.

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