Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis

Figure 2

CI/R activated XBP-1 slicing, neuroinflammation, and neuron pyroptosis in the rat MCAO model. Rats were treated with MCAO. (a) The level of Xbp-1s mRNA in each group with CI/R. (b) The level of NLRP3 mRNA in each group with CI/R. (c) The level of IL-1β mRNA in each group with CI/R. (d) The level of IL-18 mRNA in each group with CI/R. (e) Representative western blots and quantification of the indicated proteins in the hippocampal lysate from rats in each group. Relative expression was normalized to sham rats. (f–k) The expression of XBP-1s, NLRP3, Caspase-1, GSDMD-N, IL-1β, and IL-18 in the hippocampus of the four groups after CI/R injury. Each bar represents the (). , , , and were statistically significant.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)