Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis

<div>OGD/R decreased the cell viability and increased cytotoxicity, XBP-1 slicing, and inflammation in HT22 cells. The HT22 cells were, respectively, pretreated with Z-YVAD-FMK (20 <i>μ</i>M) and polyphyllin VI (4 <i>μ</i>M) 30 min before exposing to OGD/R for 24 h. (a) Cell viability was detected in HT22 cells using a CCK-8 assay. (b) LDH release was detected in HT22 cells using an LDH assay. (c) Representative western blots and quantification of the indicated proteins in the cellular lysate from HT22 cells in each group. Relative expression was normalized to that of the control group. (d–i) The expression of XBP-1s, NLRP3, Caspase-1, GSDMD-N, IL-1<i>β</i>, and IL-18 in HT22 cells after OGD/R. 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103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.05,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,55.29,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,61.53,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>).</span> All experiments were repeated for three times.</div>

Mediators of Inflammation

fig3

Figure 3

Figure 3: Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis 