Research Article
Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis
| | Gene | Primer sequences |
| | Xbp-1 spliced (forward primer) | 5-TCA GAC TAC GTG CGC CTC T-3 | | Xbp-1 spliced (reverse primer) | 5-CTC TGG GGA AGG ACA TTT GA-3 | | Xbp-1 unspliced (forward primer) | 5-CTG AGT CCG CAG CAG GTG-3 | | Xbp-1 unspliced (reverse primer) | 5-CCA CAT CCG CCG TAA AAG AAT G-3 | | NLRP3 (forward primer) | 5-CCA GAG CCT CAC TGA ACT GG-3 | | NLRP3 (reverse primer) | 5-AGC ATT GAT GGG TCA GTC CG-3 | | IL-1β (forward primer) | 5-CAG GCA GGC AGT ATC ACT CA-3 | | IL-1β (reverse primer) | 5-AGG CCA CAG GTA TTT TGT CG-3 | | IL-18 (forward primer) | 5-TCT TCA TTG ACC AAG GAA ATC GG-3 | | IL-18 (reverse primer) | 5TCC GGG GTG CAT TAT CTC TAC-3 | | GAPDH (forward primer) | 5-AGG TCG GTG TGA ACG GAT TTG-3 | | GAPDH (reverse primer) | 5-GGG GTC GTT GAT GGC AAC A-3 |
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