Nr2f2 Overexpression Aggravates Ferroptosis and Mitochondrial Dysfunction by Regulating the PGC-1α Signaling in Diabetes-Induced Heart Failure Mice

Figure 1

Overexpression of Nr2f2 aggravated the heart failure in diabetic mice. (a) Nr2f2 protein level in DCM mice was quantified by Western blot (, , , Student’ -test). (b) Nr2f2 mRNA level in DCM mice were assessed by real-time qPCR (, , , Student’ -test). (c) The overexpression efficiency of Nr2f2 was quantified by Western blot (, , , Student’ -test). (d) Fasting blood glucose level of mice at each point (AAV9-cTNT-ctrl-Con, vs. AAV9-cTNT-Nr2f2-Con, , ; AAV9-cTNT-ctrl-DIHF, vs. AAV9-cTNT-Nr2f2-DIHF, , ). (e) Body weight of mice at each point (AAV9-cTNT-ctrl-Con, vs. AAV9-cTNT-Nr2f2-Con, , ; AAV9-cTNT-ctrl-DIHF, vs. AAV9-cTNT-Nr2f2-DIHF, , ). (f) Typical echocardiography, HE staining (20x and 400x magnification) and Masson staining (400x and 630x magnification) obtained from mice. (g–j) Echocardiography analysis of EF%, FS%, LVDd (mm), and LVDs (mm). (k) Cardiomyocytes area (μm²). (l) Fibrosis area (%) (AAV9-cTNT-ctrl-Con , AAV9-cTNT-ctrl-DIHF , AAV9-cTNT-Nr2f2-Con , and AAV9-cTNT-Nr2f2-DIHF ; , two-way ANOVA, , , and , two-way ANOVA).

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