Vitronectin, a Novel Urinary Proteomic Biomarker, Promotes Cell Pyroptosis in Juvenile Systemic Lupus Erythematosus

Figure 7

VTN triggered cell pyroptosis. (a) LDH assay was performed to determine relative cell death in the indicated group in THP-1-derived macrophages; data represented the of three independent experiments; -tests were used to analyze statistical significance; , . (b) Real-time PCR was performed to examine the mRNA level of GSDMD, IL-1b, and IL-18 expression in THP-1-derived macrophages. Data represented the of three independent experiments; -tests were used to analyze statistical significance; . (c) Western blotting was performed to test pyroptosis-related proteins, including GSDMD, IL-1b, and IL-18, in THP-1-derived macrophages with or without VTN (4 μM) stimulation, and the quantified results were analyzed by the -test. Data represented the of three independent experiments; -tests were used to analyze statistical significance; . (d) The contents of IL-1b and IL-18 in the supernatant and GSDMD/GSDMD-N expression of THP-1-derived macrophages with or without VTN treatment were detected by ELISA; data represented the of three independent experiments; -tests were used to analyze statistical significance; . (e) IF assay was performed to detect GSDMD expression in THP-1-derived macrophages with or without VTN treatment.

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