Vitronectin, a Novel Urinary Proteomic Biomarker, Promotes Cell Pyroptosis in Juvenile Systemic Lupus Erythematosus

Figure 8

VTN triggered the NF-κB pathway and NLRP3 inflammasome. (a) Real-time PCR and (b) western blotting were performed to detect ASC, NLRP3, and caspase-1 expression in THP-1-derived macrophages with or without VTN treatment. Data represented the of three independent experiments; -tests were used to analyze statistical significance; , , and . (c) Phosphorylation of NF-κB was detected in THP-1-derived macrophages in response to VTN stimulation for 1 hour. (d, e) IL-18 was analyzed by western blotting and ELISA in THP-1-derived macrophages treated with VTN for 1 h, followed by BAY 11-7085 stimulation for another 48 hours. Data represented the of three independent experiments; one-ANOVA was used to analyze statistical significance; , . (f) The schematic illustration for VTN-regulated cell pyroptosis.

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