Glibenclamide Alleviates LPS-Induced Acute Lung Injury through NLRP3 Inflammasome Signaling Pathway

Figure 4

A protective role of glibenclamide in vitro inflammatory model. (a) CCK-8 assay was used to determine glibenclamide cytotoxicity (). (b) Relative NLRP3 mRNA expression in PMs was measured by real-time PCR (). (c, d) Representative images of Western blot of NLRP3 and Caspase-1/p20 in PMs and quantitative analysis (). (e) Representative immunofluorescence staining of NLRP3 in PMs. (f) Calculated percentage of NLRP3-positive nuclei (). (g–i) The level of IL-1β, IL-18, and TNF-α in cell supernatant was measured by ELISA (). Data are representative of three independent experiments (mean and SD). Scale bars, 20 μm. ns: not significant. , , , and (unpaired Student’s -test).

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