circ-Katnal1 Enhances Inflammatory Pyroptosis in Sepsis-Induced Liver Injury through the miR-31-5p/GSDMD Axis

Figure 2

Abnormal expression of circ-Katnal1 played an important role in CLP-induced sepsis. (a) Heat map showing the expression of circRNA following 12-h induction after CLP in liver tissues. (b) RT-qPCR detection showed the expression of mmu_circ_0012734, mmu_circ_0001432, mmu_circ_0012767, mmu_circ_0012771, and mmu_circ_0012774 in normal and CLP-induced mouse liver tissues. Data were presented as . ^∗∗∗, versus the sham group. (c) Genomic loci of the Katnal1 gene and mmu_circ_0001432 (circ-Katnal1). (d) The luciferase activity of circ-Katnal1 in HEK293 T cells transfected with different miRNA mimics, which were putative binding sites for the circ-Katnal1 sequence. Luciferase activity was normalized by Renilla luciferase activity. Data were presented as . ^∗∗∗. (e) RT-qPCR detection showed the expression of miR-31-5p in normal and CLP-induced mouse liver tissues. Data were presented as . ^∗∗ versus the sham group. (f, g) Prediction of the binding sites of miR-31-5p in circ-Katnal1. The MUT version of circ-Katnal1 is presented. The relative luciferase activity determined 48 h after the transfection of HEK293 T cells with miR-31-5p mimic/NC or circ-Katnal1 WT/Mut. Data were presented as . ^∗∗. (h, i) Prediction of the binding sites of miR-31-5p in 3UTR-GSDMD. The MUT version of 3UTR-GSDMD is presented. Relative luciferase activity determined 48 h after the transfection of HEK293 T cells with miR-31-5p mimic/NC or 3UTR-GSDMD WT/Mut. Data were presented as . ^∗∗∗.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)