circ-Katnal1 Enhances Inflammatory Pyroptosis in Sepsis-Induced Liver Injury through the miR-31-5p/GSDMD Axis

Figure 3

Downregulation circ-Katnal1 suppressed LPS-induced inflammatory pyroptosis in RAW264.7 cells. However inhibiting miR-31-5p or upregulating GSDMD reversed the protective effect after circ-Katnal1 silencing. (a)–(c) RT-qPCR detection showed the expression of circ-Katnal1, miR-31-5p, and GSDMD. (d)–(f) ELISA detection showed the expression of inflammatory factors TNF-α, IL-1β, and IL-6 under LPS condition (1 μg/mL). Data were presented as . ^∗, ^∗∗, ^∗∗∗, versus sham NC. ^##, ^###, versus si-circ-Katnal1 (si-mmu_circ_0001432). (g)–(j) Western blot detection showed GSDMD, caspase-1, and NLRP3 expression. Data were presented as . ^∗∗∗, versus the sham group. Data were presented as . ^∗, ^∗∗∗, versus sham NC; ^#, ^##, ^###, versus si-circ-Katnal1 (si-mmu_circ_0001432).

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