circ-Katnal1 Enhances Inflammatory Pyroptosis in Sepsis-Induced Liver Injury through the miR-31-5p/GSDMD Axis

Figure 4

Upregulation of GSDMD reversed the protective effect of miR-31-5p on LPS-induced inflammatory pyroptosis in RAW264.7 cells. (a, b) RT-qPCR detection showed the expression of miR-31-5p and GSDMD. (c)–(e) ELISA detection showed the expression of inflammatory factors TNF-α, IL-1β, and IL-6 under LPS pretreatment (1 μg/mL). Data were presented as . ^∗∗∗, versus sham NC. ^###, versus mimic. (f)–(i) Western blot detection showed the expression of GSDMD, caspase-1, and NLRP3 expression. Data were presented as . ^∗∗∗, versus sham NC. ^###, versus mimic.

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