Atractylenolide III Improves Mitochondrial Function and Protects Against Ulcerative Colitis by Activating AMPK/SIRT1/PGC-1α

Figure 6

AT III attenuates LPS-induced mitochondrial dysfunction in IEC-6 cells via AMPK/SIRT1-mediated deacetylation of PGC-1α. (a) The levels of mtDNA were examined by qPCR. (b) The MMP changes were measured and quantified by the flow cytometry assay using JC-1 probes. (c, d) The complex I and complex IV activities were measured. (e, f) The protein expression levels of PGC-1α, NRF-1, NRF-2, and Tfam were detected and quantified by Western blot. (g, h) The protein expression levels of p-AMPK, AMPK, and SIRT1 were examined and quantified by Western blot. (i, j) The acetylated levels of PGC-1α and quantification results were determined. ^$, compared to control; ^&, compared to LPS.

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