Notch Signaling Pathway Promotes Th17 Cell Differentiation and Participates in Thyroid Autoimmune Injury in Experimental Autoimmune Thyroiditis Mice

Figure 5

Flow cytometric analyses of Th17 cells in SMCs and TMCs. (a) Representative flow cytometry results of Th17 cells in SMCs in the three groups. (b) Representative flow cytometry results of Th17 cells in SMCs from EAT-A mice treated with different concentrations of DAPT in vitro. (c) Representative flow cytometry analyses of Th17 cells in TMCs from EAT-A mouse thyroids. (d) Summary of Th17 cell proportions. There were significant differences of Th17 cells in SMCs among the three groups (Welch , , Welch’s ANOVA). Additionally, Th17 cell percentages in EAT-A mice were significantly higher compared to those in NC mice ( vs. , , Tamhane’s T2 test). In EAT-B mice, DAPT treatment via intraperitoneal injection significantly decreased the percentage of Th17 cells () compared to EAT-A mice (, Tamhane’s T2 test). (e). Summary of Th17 cell percentages in four groups with different DAPT concentrations in vitro. Analyses revealed that Th17 cell percentages decreased in a concentration- dependent manner (, , one-way ANOVA).

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