IFN-α-2b Reduces Postoperative Arthrofibrosis in Rats by Inhibiting Fibroblast Proliferation and Migration through STAT1/p21 Signaling Pathway

Figure 5

The inhibitory effect of IFN-α-2b on the proliferation of fibroblasts by STAT1/P21 signaling pathway. (a) After treating fibroblasts with IFN-α-2b or combined with fludarabine, perform EdU staining to analyze the result image. The cells shown in red are marked as positive. (b) The results of the EdU incorporation test showed that as the concentration of IFN-α-2b increased, the percentage of positive cells decreased significantly. This trend was partially reversed by fludarabine. (c, e) Western blotting shows the increase of PCNA, cyclin A, and collagen I and predicts the expression levels of related pathway proteins. (d, f) The analysis results showed that IFN-α-2b significantly reduced the relative expression levels of PCNA, cyclin A, and collagen I in a concentration-dependent manner, while increasing the relative expression levels of P-STAT1 and P21. And after fludarabine application, the trend of action was partially reversed. The histogram shows the results of three repeated detections of the gray value of the Western blot band. Compared with the control group. Compared between the two groups, ().

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