HMGB2 Deficiency Mitigates Abdominal Aortic Aneurysm by Suppressing Ang-II-Caused Ferroptosis and Inflammation via NF-κβ Pathway

Figure 6

Silencing of HMGB2 suppressed Ang-II-stimulated AAA formation and ferroptosis in vivo. Mice were injected with Ang-II to establish an AAA model. RT-qPCR and western blot assays were used to measure HMGB2 level (a and b). Immunofluorescence staining was utilized to measure HMGB2 and α-SMA expression (c). The representative images of aorta in Sham, Ang-II, Ang-II + shNC, and Ang-II + shHMGB2#3 groups (d). GPX4, FTH1, ACSL4, and COX2 protein levels were measured by western blot (e). Typical images showing HE staining for aortic cross-sections (f). ELISA assay was performed to detect IL-6 and IL-1β level (g).

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