Amphiregulin Induces iNOS and COX-2 Expression through NF-κB and MAPK Signaling in Hepatic Inflammation

Figure 1

Hepatic inflammation and AREG expression were increased in mice fed an MCD diet for 2 weeks. (a) Levels of alanine aminotransferase and aspartate aminotransferase were evaluated in the plasma of mice fed a normal diet or an MCD diet. (b) The mRNA levels of TNF-α, IL-6, IL-1β, and IL-8 were measured in the liver by real-time PCR. (c) Expression of L3T4, F4/80, and LyG6 mRNA in the liver was determined using real-time PCR. (d) Immunohistochemical staining with anti-AREG was performed on the livers of mice. Representative images and percentage of AREG + cells. Scale bar: 50 µm. (e) The mRNA level of AREG was measured in the liver by real-time PCR. compared to the ND.

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