Research Article

The Spliceosome Factor EFTUD2 Promotes IFN Anti-HBV Effect through mRNA Splicing

Figure 2

IFN anti-HBV activity was restricted in EFTUD2+/-HepG2.2.15 cells. WT and EFTUD2+/-HepG2.2.15 cells were maintained in DMEM with or without IFNα-2b (5000 IU/mL) for 9 days. (a–c) The supernatants were then collected. HBV DNA was evaluated by real-time PCR, and HBeAg and HBsAg were detected by ECIIA. from three independent experiments was shown. Student’s -test was used to determine the significance of differences between the two groups. , and . (d) Cells were immunostained with antibodies against HBV capsid protein (HBcAg) and visualized under fluorescence microscopy (20x). Nuclei were counterstained with DAPI. The image shown represents three different microscopic fields; the percentage of HBcAg-positive cells was indicated ().
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