The Spliceosome Factor EFTUD2 Promotes IFN Anti-HBV Effect through mRNA Splicing

Figure 3

Decreased expression of EFTUD2 suppresses the expression of IFN-α downstream antiviral effectors. (a) Both EFTUD2^+/-HepG2.2.15 cells and WT HepG2.2.15 cells were treated with 5000 IU/mL IFN-α for 24 h. IFN-α-induced Mx1, OAS1, and PKR expression levels were measured by Western blotting. (b) HepG2.2.15 cells were transfected with si-EFTUD2 or si-NC for 24 h and then treated with 5000 IU/mL IFN-α for 24 h. The mRNA protein levels of Mx1, OAS, and PKR were analyzed by Western blotting after 48 h of transfection. The experiments were repeated three times each.

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