Research Article
The Spliceosome Factor EFTUD2 Promotes IFN Anti-HBV Effect through mRNA Splicing
Figure 4
EFTUD2 upregulated Mx1, OAS1, and PKR expressions by pre-mRNA splicing. Both EFTUD2+/-HepG2.2.15 and WT HepG2.2.15 cells were treated with 5000 IU/mL IFN-α for 8 h. We compared Mx1, OAS1, and PKR transcript mRNA expressions between EFTUD2+/-HepG2.2.15 cells and WT HepG2.2.15. (a) Mx1-201 mRNA levels. (b) OAS1-202 mRNA levels. (c) EIF2AK2-203 mRNA levels. (d) Mx1-210 mRNA levels. (e) OAS1-205 mature mRNA levels. (f) EIF2AK2-205 mRNA levels. Mx1-201, OAS1-202, and EIF2AK2-203 are protein encoding transcripts, and Mx1-210, OAS1-205, and EIF2AK2-205 are nonprotein encoding transcripts based on Ensembl bioinformatics. from three independent experiments performed in triplicate is shown. Student’s -test was used to determine the statistical difference between the two groups. , and .
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