IgE Immune Complexes Mitigate Eosinophilic Immune Responses through NLRC4 Inflammasome

Figure 3

Ova-IgE ICs suppress NLRC4 inflammasome-mediated inflammation in EoL-1 cells. EoL-1 cells were treated with IgE (10 µg)-Ova (1 µg) immune complexes. For NLRC4 inflammasome induction, TLR2-primed EoL-1 cells were transfected with 100 ng/ml FLA and incubated for 20hr. (a) Western blot and corresponding densitometry analyses of cell lysates (30 µg). NLRC4, caspase-1, and IL-1β protein expressions were immunoblotted. Values represent the mean ± SD and are representative of three separate experiments. (b) Supernatants were collected and analyzed for IL-1β and (c) IL-10 by ELISA. Experiments were carried out in duplicate. Values represent the mean ± SD and are representative of two separate experiments. by Student’s t-test. , , and .

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