IgE Immune Complexes Mitigate Eosinophilic Immune Responses through NLRC4 Inflammasome

Figure 4

Ova-IgE ICs reduced MMP2, MMP9, TIMP1, and TIMP2 proteins but not MMP9 secretion and activation. EoL-1 cells were treated with IgE (10 µg)-Ova (1 µg) immune complexes. For NLRC4 inflammasome induction, TLR2-primed EoL-1 cells were transfected with 100 ng/ml FLA and incubated for 20 hr. Western blot and corresponding densitometry analyses of cell lysates (30 µg). (a) MMP2, MMP9, TIMP1, and TIMP2 protein expressions were immunoblotted. Values represent the mean ± SD and are representative of three separate experiments. (b) Pro- and active MMP9 enzyme activities from the supernatants of the stimulated and nonstimulated cells were measured by gelatin zymography assay.

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