Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification

<div>The effects of GDFMD on autophagy and PI3K/Akt/mTOR pathway-related proteins in the liver of mouse models of WD. Expression of LC3 and Beclin-1 was observed using immunofluorescence staining (a); the protein levels of p-PI3K, PI3K, p-AKT, AKT, p-mTOR, mTOR, Beclin-1, p62, and LC3-II/LC3-I ratio (b) in liver tissues of mice from the normal, model, Gandoufumu (high dose, low dose, and middle dose), and penicillamine groups. 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Mediators of Inflammation

fig4

Figure 4

Figure 4: Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification 

Figure 4 | Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification 