Betulinic Acid Attenuates Osteoarthritis via Limiting NLRP3 Inflammasome Activation to Decrease Interleukin-1β Maturation and Secretion

Figure 9

BA inhibited the activation of NLRP3 inflammsome. (a–c, f) LPS-primed BMDMs were treated with ATP or ATP + BA for 1 hr. (a) The cytokines of IL-1β and (b) IL-18 in supernanants were measured by ELISA. (c) Representative western bolt image of caspase-1 and IL-1β. (f) The quantification of active caspase-1 (P20) and active IL-1β (P17). (d, e) PMA-induced THP-1 macrophages were primed with LPS for 4 hr and then stimulated with ATP or ATP + BA for 1 hr. (d) Representative western bolt image of Caspase-1 and IL-1β. (e) The quantification of active caspase-1 (P20) and active IL-1β (P17). . (n = 3).

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