Curcumin Improves Diabetic Cardiomyopathy by Inhibiting Pyroptosis through AKT/Nrf2/ARE Pathway

Figure 5

Interfering with the expression of Nrf2 hinders the cardioprotective effect of curcumin. Glu: glucose; Cur: curcumin; the dose of curcumin used in cell experiments is 14 μM. Si-Nrf2: interference virus vector targeting the Nrf2 gene. (a) PCR and western blotting detection of intracellular Nrf2 expression. (b) Western blotting detection of pyroptosis protein expression levels. (c) Western blotting detection of protein expression levels in the AKT/Nrf2 pathway. (d) Immunofluorescence detected the fluorescence intensity of NLRP3 (20x). (e) Changes in the mitochondrial membrane potential levels of cardiomyocytes. The stronger the red fluorescence, the worse the cell viability; the stronger the green fluorescence, the better the cell viability (20x). (f) TUNEL fluorescent probe to detect the incidence of apoptosis (20x). (g) The CCK-8 test detects cardiomyocyte activity. , high-glucose group vs. normal group and curcumin-treated group. #: transfected siRNA vs. untransfected cells (in high-glucose medium). #: transfected siRNA vs. untransfected cells (in high-glucose medium). Values are expressed as the deviation.

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