lncRNA MIR503HG Targets miR-191-5p/PLCD1 Axis and Negatively Modulates Apoptosis, Extracellular Matrix Disruption, and Inflammation in Abdominal Aortic Aneurysm

Figure 1

miR-191-5p augment influenced behaviors of VSMCs in AAA. (a) AAA tissues and control tissues were obtained for measurement of miR-191-5p level through qRT-PCR. (b) qRT-PCR data revealed the overexpression efficiency of miR-191-5p mimics in VSMCs. (c, d) CCK-8 and EdU staining assays were conducted for detecting the viability of VSMCs under miR-191-5p overexpression. (e–g) miR-191-5p mimic influence on VSMC apoptosis was assayed via flow cytometry, TUNEL staining, and caspase-3 activity detection assays. (h, i) Western blotting for the proliferation/apoptosis-associated proteins and proteins involved in ECM degradation after miR-191-5p mimic transfection. (j, k) Serum levels of the TNF-α and IL-6 were quantified by ELISA kits in VSMCs. Three biological replicates were involved for each experiment. .

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