lncRNA MIR503HG Targets miR-191-5p/PLCD1 Axis and Negatively Modulates Apoptosis, Extracellular Matrix Disruption, and Inflammation in Abdominal Aortic Aneurysm

Figure 2

MIR503HG sponged miR-191-5p in VSMCs. (a) Pull-downs of biotinylated probes were quantified by qRT-PCR. (b, c) miR-191-5p binding sites on MIR503HG sequence (wild or mutant) were cloned into pmirGLO Vector for luciferase reporter assays. (d) MIR503HG expression in AAA tissues versus control samples was shown. (e) MIR503HG and miR-191-5p expression correlation was determined by Spearman’s correlation analysis. (f) qRT-PCR was done for the silencing efficiency of shMIR503HG. (g) The miR-191-5p level in response to MIR503HG depletion was analyzed. (h, i) VSMC viability responding to shMIR503HG transfection was tested. (j–l) VSMC apoptosis after shMIR503HG transfection was analyzed. (m, n) The levels of proliferation/apoptosis-associated proteins and proteins involved in ECM degradation after silencing MIR503HG were evaluated. (o, p) Serum levels of inflammatory cytokines in shMIR503HG-regulated VSMCs were analyzed. Three biological replicates were involved for each experiment. and .

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