lncRNA MIR503HG Targets miR-191-5p/PLCD1 Axis and Negatively Modulates Apoptosis, Extracellular Matrix Disruption, and Inflammation in Abdominal Aortic Aneurysm

Figure 3

miR-191-5p targeted PLCD1 in VSMCs. (a) 13 possible mRNAs of miR-191-5p were screened out and presented as the Venn diagram. (b) Change in luciferase activity revealed the interplay of miR-191-5p and 13 possible mRNAs. (c) PLCD1 enrichment in pull-down complex by biotinylated miR-191-5p was measured. (d) PLCD1 levels in AAA tissues compared to the control group were measured. (e) Correlation between expressions of PLCD1 and miR-191-5p or MIR503HG was analyzed. (f, g) PLCD1 level in differentially transfected VSMCs was analyzed. (h–l) The regulation of PLCD1 knockdown on VSMC viability and apoptosis was assessed. (m, n) The levels of proliferation/apoptosis-associated proteins and proteins involved in ECM degradation under PLCD1 depletion were assessed. (o, p) Serum levels of inflammatory cytokines in shPLCD1-regulated VSMCs were measured. Three biological replicates were involved for each experiment. .

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