Cell-Specific Regulation of Inflammatory Cytokines and Acute-Phase Proteins by the Glucocorticoid Receptor

Figure 3

Cellular mRNA and corresponding culture-media protein levels, donor 1. Cells were treated with TNFα 50 ng/mL, LPS 10 ng/mL, and/or dexamethasone 0.1 or 1 µM for 24 hr. Gene expressions of (a) CXCL10, (b) PAI-1, and (c) HAMP were assessed by qPCR and normalized to AKIRIN1 mRNA. Corresponding protein levels (d–f) were assessed by ELISA assays of culture media. Data for HAMP (Hepcidin) was log10 transformed for statistical analysis and graphing. Mean ± SE, n = 3 replicate wells. , , and . ns, not significantly different.

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