Cell-Specific Regulation of Inflammatory Cytokines and Acute-Phase Proteins by the Glucocorticoid Receptor

Figure 6

Chemokine protein levels in cellular lysates of primary human hepatocytes, donor 2. Cells were treated with TNFα 50 ng/mL, LPS 10 ng/mL, dexamethasone 1 µM, and RU486 5 µM for 24 hr, frozen, then lysed in RIPA buffer for ELISA assays of (a) CXCL1, (b) CXCL2, and (c) CXCL8. CXCL8 data was log10 transformed for statistical analysis due to large error bars. Mean ± SE, n = 3–4-well replicates, and , , and . ns, not significantly different.

(a)

(b)

(c)